Concerning Criteria for transfer of Attenuated Yersinia pestis strains from Pathogenicity (Hazard) Group I into Pathogenicity Group III

Live vaccines induce both cellular and humoral immunity are cheap easy to use. The induction of is provided through the reproduction vaccine strain in host body without development disease, since bacterium which it necessary characterized by reduced virulence (attenuation). first generation attenuated strains was chosen from a variety spontaneous or physically, chemically biologically induced mutants after assessment. rapid molecular genetics makes possible significantly reduce time pathogen attenuation via obtaining knockout with genes selected researcher inserting “avirulence genes” into genome. But, given that methodological aspects design avirulent basically clarified, absence officially established criteria for assessing hazard regulatory documents hinders determination degree attenuation. In this regard, there need changes procedure accounting storage bacterial cultures, as well regulation process transferring plague 1 st 3 rd pathogenicity group subsequent use preparations development. Thereat, requirements safe Yersinia pestis testing loss should be maintained at high levels